bedGraph format

The official definition of bedGraph can be found here. As for all other files generated directly from MACS3, you may need to add a trackline to the beginning of the file when you upload the file to UCSC genome browser for display. A minimal trackline for bedGraph is track type=bedGraph. Please read the above link to the official definition page for more instruction. The bedGraph format file can be further converted into a binary format named ‘bigWig’ for smaller file size and for fast and random access. Check this gist for a script to convert the plain text bedGraph to binary bigWig.